HIGHLY SENSITIVE LABEL-FREE ELECTROCHEMICAL DETECTION OF HEAT SHOCK PROTEIN WITH LOW-COST SCREEN-PRINTED ELECTRODES

Heat shock proteins are produced when the organisms exposed to various environmental stress conditions such as temperature, light, toxins. It is a known fact that in bacteria, which has HSP gene, antibiotics can trigger expression of heat protein. However, response protein genes not been fully clarified literature yet, studies still ongoing. In this study, novel way for detection protein65 was investigated using electrochemical impedance spectroscopy due its sensitivity, selectivity, low cost. To do so, probe and target were designed hybridization behavior with studied upon binding onto screen-printed electrodes. Cyclic voltammetry performed analyze surface characterization secreen printed electrodes performance screen tested electrocehimcal by measuring changes charge transfer resistance hybridization. Based on obtained results, confirmed an appropriate increase values compliance proved be effectively used detect heast label-free. Results presented here lead development antibiotic susceptibility assay based future.